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* Investigators say defence chief refused to answer questions
* Ex-minister Serdyukov a witness in $100 million fraud case
* Federal investigators warn Serdyukov could become suspect
By Gabriela Baczynska
MOSCOW, Jan 11 (Reuters) - Russian investigators accused former Defence Minister Anatoly Serdyukov on Friday of trying to obstruct their inquiry into a suspected $100 million fraud, the biggest corruption scandal of President Vladimir Putin's third term.
Serdyukov was fired by Putin in November after more than five years in office and is now a witness in a case in which a former subordinate, Yevgeniya Vasilyeva, is accused of fraud and embezzlement over deals involving Defence Ministry property.
Vasilyeva, 33, was charged in connection with a string of cut-price deals in which, investigators say, prime real estate was sold off to insiders at a loss to the taxpayer of at least 3 billion roubles ($98 million).
She was found to have paintings, rare antiques and more than 100 expensive rings when her apartment was raided in October.
The former minister, seen as a loyal Putin ally in years heading the Russia's tax inspectorate and the military, was summoned by the federal Investigative Committee for the second time and refused to answer questions, the committee said.
It said Serdyukov and his lawyer had "informally laid out their version of events in a way that benefits them, but did not answer uncomfortable questions of substance".
In a statement, it said Serdyukov was within his rights to decline to answer questions but that it prompted "puzzlement at the least" and warned that he could at some point become a suspect.
"In the given situation, the stance of the former Defence Minister can be regarded by the investigation as his effort to hinder the inquiry," the committee said.
The committee said it was "entirely possible" his status could be changed from witness to suspect.
It is extremely rare for a former Russian government minister to be prosecuted in a criminal case since the collapse of the Soviet Union in 1991.
Putin has made renewed promises to crack down on corruption in his new six-year term, which began in May, but critics doubt he is committed and say graft has worsened since he came to power in 2000.
Serdyukov, 51, made no comment as he left the Investigative Committee headquarters in Moscow. Vasilyeva, who is under house arrest in her large Moscow apartment, has not spoken publicly about the charges.
Russia ranked 133rd, alongside Honduras and Guyana, out of 174 states in Transparency International's 2012 Corruption Perception Index.